A unique combination of physical properties has established DMSO as the standard in cell preservation. In 1959 it was discovered that dimethyl sulfoxide (DMSO), which passes through cell membranes more readily than glycerol, is also useful as a cryoprotectant. When combined with water, DMSO exhibits unique and interesting thermodynamic properties. The eutectic mixture of the combination results in a liquid at minus 80° C!

DMSO penetrates cell membranes and depresses the freezing point of contained water. As the temperature decreases in the cryopreservation cycle, the cell is protected from freezing and the deleterious effects of ice crystals. DMSO is used in the cryopreservation of cells and biological tissue for a variety of end use applications. These include tissue banks, vaccines, drug delivery applications, cell culture and in laboratory cell testing.

 

Related Resources

DMSO USP Product Sheet: Representative specification, packaging and basic uses

DMSO section: Portion of the webpage dedicated to general DMSO topics

DMSO USP page: Describes uses for DMSO USP

DMSO in the Pharmaceutical Industry: More about the use of DMSO “From Benchtop to Bedside”